کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246506 969758 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk
چکیده انگلیسی

A pretreatment-free immunochromatographic assay for detection of chloramphenicol (CAP) in milk was developed. The assay is based on competition between CAP molecules in the sample and immobilized CAP–protein conjugate for binding to monoclonal anti-CAP antibodies conjugated with colloidal gold particles (average diameter 30 nm). The assay is carried out in the course of sample flowing along test strip with immobilized reactants, and its results can be detected by the naked eye or by a photometric device. Effect of the concentration of immunoreactants on assay characteristics was studied. The assay protocol with maximal sensitivity and reliability was optimized using measured values of brightness of lines. Detection limit for CAP is 10 ng mL−1. Assay duration is 10 min, and it can be carried out at room temperature without any additional devices and reactants. The developed test strip has been applied to CAP detection in dairy products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 81, Issue 3, 15 May 2010, Pages 843–848
نویسندگان
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