Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation

• Quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS.
• Enrichment at the peptide level after proteolytic digestion.
• Chemical derivatization of peptides may enhance ionization efficiency.
• Sample-preparation aspects of quantifying biopharmaceutical proteins.
• Automating the entire analytical procedure.

Part I of this review discusses sample-preparation aspects of quantifying biopharmaceutical proteins in complex biological matrices by LC-MS/MS with a focus on blood-derived body fluids. We conclude Part I with a short overview over options for automating the entire analytical procedure, which is indispensable for routine applications in pharmacokinetic and clinical studies.