Tackling the increasing complexity of therapeutic monoclonal antibodies with mass spectrometry

• We highlight the use of mass spectrometry for the analysis of therapeutic mAbs.
• We focus on mass spectrometry for characterization of increasingly complex samples.
• Hot topics are sequence variances, biosimilars, bispecific mAbs, and mixtures of mAbs.
• We report on new instrumentation to improve the analysis of therapeutic antibodies.

Mass spectrometry (MS) is emerging as an efficient method for the structural analysis of various new antibody-based therapeutic products. Here, we review the current trends and describe recent applications that highlight the use of MS to tackle the increasing complexity of monoclonal antibodies.