Recent advances in pharmaceutical separations with supercritical fluid chromatography using chiral stationary phases

Supercritical fluid chromatography (SFC) has developed rapidly in recent years, particularly in the area of enantioseparations. The merits of SFC (e.g., fast analysis speed, wide polarity compatibility, lower cost of the mobile phase and higher column efficiency) would in many cases make it a better choice than high-performance liquid chromatography in drug discovery for the pharmaceutical industry.Packed-column SFC meets the gradual rise in demand for simultaneous chiral and achiral separations, impurity quantitation and direct scaling up from the pharmaceutical purifications achieved in the laboratory, especially for chiral drugs.This review focuses mainly on the latest examples of pharmaceutical separations with chiral stationary phases in SFC for efficient analyses and preparative-scale purifications.

► We overview SFC applications for analytical and preparative chiral separation.
► We summarize the implementation of CSPs with packed-column SFC.
► We overview the SFC conditions for optimization of enantioseparation.
► We summarize tandem SFC for chiral separation.