کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1248589 970446 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins
چکیده انگلیسی

Speciation of heteroelement-containing proteins essential for human health is one of the most rapidly developing areas in bio-inorganic analytical chemistry (metallomics). We discuss recent advances in mass spectrometry (MS) techniques for detection and identification of selenium (Se)-containing proteins separated by gel electrophoresis (GE). These advances include location of Se-containing bands in 1D GE or spots in 2D GE by laser-ablation inductively coupled plasma MS (LA-ICP-MS) followed by protein identification by matrix-assisted laser-desorption/ionization MS (MALDI-MS) and nano-high-performance liquid chromatography-electrospray-tandem MS (nanoHPLC-ESI-MS2). We highlight the differences with the classical proteomics approach resulting from the presence of the Se atom.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 26, Issue 3, March 2007, Pages 183–190
نویسندگان
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