Determination of flavonoids and their metabolites by chromatographic techniques

• Biological properties and biotransformation of flavonoids and their metabolites.
• Different methods for sample preparation of flavonoids and their metabolites.
• Analytical methods for identification of flavonoids and their metabolites.

This article presents an overview of biological properties and biotransformation of isoflavones in vivo and describes methods of their identification, with special attention given to investigation by liquid chromatography.We discuss the advantages and the disadvantages of different methods of sample preparation, and the choice of suitable columns, solvents and detectors used for identifying isoflavones in different samples.