Stationary phases for separation of nucleosides and nucleotides by hydrophilic interaction liquid chromatography

• State-of-art and recent trends in the development of novel materials for HILIC.
• Monolith-column technology applied to HILIC separation.
• HILIC performance is discussed in terms of separation, sensitivity and speed increase.
• The review focuses on the separation of nucleobases, nucleosides and nucleotides.
• We review applications of HILIC in biology, the food industry and medicine.

This review describes recent trends in novel stationary phases for hydrophilic interaction liquid chromatography (HILIC), focusing on separation of nucleosides and nucleotides. The performance of these novel HILIC stationary-phases is discussed in terms of resolution, separation, improved sensitivity and increased speed in order to achieve more efficient, faster chromatographic separations.