کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1249375 970642 2009 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioanalytical applications of aptamer and molecular-beacon probes in fluorescence-affinity assays
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Bioanalytical applications of aptamer and molecular-beacon probes in fluorescence-affinity assays
چکیده انگلیسی

This critical review summarizes recent developments in highly sensitive, specific assays using nucleic-acid (NA)-affinity probes and fluorescence detection. We emphasize two groups of DNA and RNA probes (i.e. aptamers and molecular beacons) because of the increase in their bioanalytical applications. The affinity and the specificity of these NA probes combined with the diverse detection capability of fluorescence measurements (e.g., intensity, polarization, resonance-energy transfer and decay life-time) enable ultrasensitive assays for proteins, gene mutations, single-nucleotide polymorphisms and molecular-binding events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 28, Issue 7, August 2009, Pages 878–892
نویسندگان
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