کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1250284 | 970803 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The auxiliary determination of the binding site of berberine binding to human serum albumin by surface-enhanced Raman scattering
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The auxiliary determination of the binding site of berberine binding to human serum albumin by surface-enhanced Raman scattering The auxiliary determination of the binding site of berberine binding to human serum albumin by surface-enhanced Raman scattering](/preview/png/1250284.png)
چکیده انگلیسی
We report on the joint application of fluorescence, ultraviolet-visible (UV-Vis) and Raman spectroscopy to the study of berberine with human serum albumin (HSA). We propose the surface-enhanced Raman scattering (SERS) technique to improve the understanding of the quenching interaction caused by berberine which could be applied in recognition process of fluorescent drugs with large biomolecules. The fluorescence and UV-Vis spectroscopic results show that the fluorescence intensity of HSA is significantly decreased in the presence of berberine, and the quenching mechanism is static. The SERS technique demonstrates clear advantages over direct measurements in physiological conditions. By means of this method, we are able to deduce important information concerning the binding property of berberine when interacting with HSA. We show the nitrogen atom is free but the dioxolane is involved in the spontaneously electrostatic inducement and subsequently hydrophobic binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 55, Issue 1, 18 January 2011, Pages 65-68
Journal: Vibrational Spectroscopy - Volume 55, Issue 1, 18 January 2011, Pages 65-68
نویسندگان
Wei Zhang, Yu Zhao, Xueyuan Bai, Ge Hui, John R. Lombardi, Daqing Zhao, Bing Zhao,