کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251810 | 1496310 | 2012 | 6 صفحه PDF | دانلود رایگان |
The decomposition of hydrogen peroxide catalyzed by catalase entrapped in the pool of di-palmitoylphosphatidyl choline unilamellar liposomes has been studied. The rate of the process was evaluated by following the production of oxygen as a function of time. Under the experimental conditions employed the rate of oxygen production was controlled by the diffusion of hydrogen peroxide, allowing for the estimation of the diffusion coefficient of hydrogen peroxide across the liposome bilayer.The rate of diffusion across the bilayer increases with the temperature and the presence of fluidizers (n-nonanol), according with changes in the bilayer fluidity, as sensed by 1,6-diphenyl hexatriene (DPH) fluorescence anisotropy. A peculiar aspect of the data is the fast hydrogen peroxide diffusion observed at the bilayer phase transition temperature. This fast diffusion is associated to rafts fluctuations that take place in the partially melted bilayer. These fluctuations have no effect on the microviscosity sensed by DPH.
The maximum rate of HOOH penetration takes place at the transition temperature.Figure optionsDownload as PowerPoint slideHighlights
► Entrapped catalase decomposes externally added hydrogen peroxide.
► The rate of the process follows the bilayer microviscosity and rafts fluctuation.
► Maximum entrance rate takes place at the bilayer transition temperature.
Journal: Chemistry and Physics of Lipids - Volume 165, Issue 6, September 2012, Pages 656–661