کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1253755 1496361 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
1H NMR investigation on interaction between ibuprofen and lipoproteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
1H NMR investigation on interaction between ibuprofen and lipoproteins
چکیده انگلیسی

A large number of studies indicate that oxidative modification of plasma lipoproteins, especially low-density lipoprotein (LDL), is a critical factor in initiation and progression of atherosclerosis. We have previously found that ibuprofen (IBP), a potential antioxidant drug to inhibit LDL oxidation, interacted with lipoproteins in intact human plasma. In the present study, we compare the binding affinities of IBP to LDL and HDL (high-density lipoprotein) by 1H NMR spectroscopy. When IBP is added into the HDL and LDL samples, the N+(CH3)3 moieties of phosphatidylcholine (PC) and sphingomyelin (SM) in lipoprotein particles experience the chemical shift up-field drift. Intermolecular cross-peaks observed in NOESY spectra imply that there are direct interactions between ibuprofen and lipoproteins at both hydrophobic and hydrophilic (ionic) regions. These interactions are likely to be important in the solubility of ibuprofen into lipoprotein particles. Ibuprofen has higher impact on the PC and SM head group (N+(CH3)3) and (CH2)n group in HDL than that in LDL. This could be explained by either IBP has higher binding affinity to HDL than to LDL, or IBP induces orientation of the phospholipid head group at the surface of the lipoprotein particles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 148, Issue 2, August 2007, Pages 105–111
نویسندگان
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