کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1316268 1499456 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro
ترجمه فارسی عنوان
شناسایی دی هیدرات دی کالیسیوم فسفات ذخیره شده در طی استخراج استئوبلاست در محیط کشت
کلمات کلیدی
استئوبلاست ها، معدنی شدن، دی کالیسیک فسفات دهیدراته، اکسا کلسیوم فسفات
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
چکیده انگلیسی

Highlight
• Features comparable to DCPD and OCP were detected in osteoblast mineralization.

The hydroxyapatite (HAP) with variable chemical substitutions has been considered as the major component in the mineralized part of bones. Various metastable crystalline phases have been suggested as transitory precursors of HAP in bone, but there are no consensuses as to the nature of these phases and their temporal evolution. In the present study, we cultured rat calvarial osteoblasts with ascorbate and β-glycerophosphate to explore which calcium phosphate precursor phases comprise the initial mineral in the process of osteoblast mineralization in vitro. At the indicated time points, the deposited calcium phosphate was analyzed after removing organic substances from the extracellular matrix with hydrazine. The features comparable to dicalcium phosphate dihydrate (DCPD) and octacalcium phosphate (OCP), in addition to HAP, were detected in the mineral phases by high resolution transmission electron microscopy. And there was a trend of conversion from DCPD- and OCP-like phases to HAP in the course of mineralization, as indicated by Fourier-transform infrared microspectroscopy, energy-dispersive X-ray spectroscopy and synchrotron X-ray powder diffraction analyses. Besides, biochemical assay showed a progressive decrease in the ratio of mineral-associated proteins to calcium with time. These findings suggest that DCPD- and OCP-like phases are likely to occur on the course of osteoblast mineralization, and the mineral-associated proteins might be involved in modulating the mineral phase transformation.

This study provided new details of apatite crystal growth mechanisms that HAP in mineral deposition by osteoblast cultures were found to yield partly from DCPD-like and OCP-like phase as the intermediates. In addition, the results indicated that mineral associated proteins might be essential factors to mediate those inorganic phase transformations.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 131, February 2014, Pages 109–114
نویسندگان
, , , , , ,