کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1317079 | 1499481 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Interaction of iron(II)-heme and artemisinin with a peptide mimic of Plasmodium falciparum HRP-II Interaction of iron(II)-heme and artemisinin with a peptide mimic of Plasmodium falciparum HRP-II](/preview/png/1317079.png)
The interaction of heme or heme-artemisinin adducts (heme-art) with different peptides mimicking repeat sequences of the Histidine-Rich-Protein-II of Plasmodium falciparum (PfHRP-II) was investigated. The pseudo-first order rate constants of the coordination of heme or heme-art onto a histidine rich peptide, used as a mimic of PfHRP-II putative heme binding sequence, are of the same order of magnitude, namely 42 and 14 s−1, respectively. Despite the intrinsic reactivity of the carbonyl at C10 of heme-art toward a hydroxyl function, a peptide containing a serine or threonine residue does not readily react with heme-art adducts. Therefore, a much higher affinity of heme-art compared to heme toward PfHRP-II, if so, must be induced by a specific interaction or a chemical reaction, these phenomena being both due to the tertiary structure of the parasite protein itself.
Journal: Journal of Inorganic Biochemistry - Volume 101, Issues 11–12, November 2007, Pages 1739–1747