کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1317874 976599 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-intercalative binding mode of bridged binuclear chiral Ru(II) complexes to native duplex DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Non-intercalative binding mode of bridged binuclear chiral Ru(II) complexes to native duplex DNA
چکیده انگلیسی

A pair of chiral binuclear ruthenium(II) complexes were prepared and their binding affinities towards double stranded native DNA were assessed by observing isotropic absorption, polarized light spectra – circular and linear dichroism (CD and LD), fluorescence quenching and DNA thermal denaturation. Upon binding to DNA, the complexes produced LD signals consisting of positive and negative signals in the absorption region, although they exhibited red shift and hypochromism in the absorption spectrum. These contrasting observations indicated that the binding modes of the complexes are largely deviated from classical intercalative binding. Groove binding of the complexes to DNA was found to be more likely than intercalative binding. The small increase of DNA melting temperature in the presence of the complexes indicated a predominance of DNA groove binding. The absence of “molecular light switch effect” further supported non-intercalative binding. The groove binding propensity of complexes was also supported by comparison of the resulting data with the [Ru(phen)2(dppz)]2+.

Providing a rotational freedom to the bridge moiety of bis-Ru(II) complex prevent intercalation.Figure optionsDownload as PowerPoint slideHighlights
► Bis-Ru(II) complexes with 4,4′-biphenylene(imidazo[4,4-f] [1] and [10]phenanthroline) bridge were synthesized.
► Binding mode to DNA were studied by various methods.
► It is not classically intercalated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 105, Issue 12, December 2011, Pages 1569–1575
نویسندگان
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