کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1323069 | 1499844 | 2016 | 12 صفحه PDF | دانلود رایگان |

• Some organometallic Ru(II), Rh(III) and Ir(III) complexes are explored as anticancer agents.
• DNA and protein binding has been established by UV–vis and fluorescence studies.
• These interactions have been supported by DFT and Molecular docking studies.
• In vitro anticancer activities towards SiHa cell line have been investigated.
Synthesis, characterization, DNA and protein binding as well as anticancer activity of the organometallic complexes [(η6-C6H6)RuCl(APBI)]Cl (1), [(η6-p-MeC6H4Pri)RuCl(APBI)]Cl (2), [(η6-C6Me6)RuCl(APBI)]Cl (3), [(η5-C5Me5)RhCl(APBI)]Cl·H2O (4) and [(η5-C5Me5)IrCl(APBI)]Cl·H2O (5) containing 2-aminophenyl benzimidazole (APBI) have been described. The complexes 1–5 exhibited strong DNA, protein binding and anticancer activity against cervical cancer (SiHa) cell line. Their binding with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) have been examined by absorption and emission spectral studies. Strong interactions between complexes and CT-DNA have been affirmed by absorption spectral and EthBr displacement studies, while interaction with BSA via static quenching explored by fluorescence titration, synchronous and 3D fluorescence spectroscopy. The interactions between 1–5 and DNA has also been scrutinized by 1H NMR spectral studies using guanosine as a model for DNA. These results have been supported by DFT calculations and molecular docking studies. Cytotoxicity, apoptosis and in vitro anticancer activity of 1–5 toward SiHa cell line have been investigated by MTT assay and acridine (AO)/ethidium bromide (EthBr) fluorescence staining. Overall results revealed that DNA and protein binding, as well as anticancer activity of 1–5 follows the order as 5 > 3 > 2 > 1 > 4.
Synthesis, characterization, DNA and protein binding as well as anticancer activity of organometallic Ru(II), Rh(III) and Ir(III) complexes (1–5) based on 2-(1H-benzo[d]imidazol-2-yl)aniline have been described. Strong DNA and protein binding affinity for 1–5 has been deduced from various spectral studies and supported by theoretical calculations. These complexes display excellent cytotoxicity and in vitro anticancer activity against SiHa cell line.Figure optionsDownload as PowerPoint slide
Journal: Journal of Organometallic Chemistry - Volume 801, 1 January 2016, Pages 68–79