کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1357829 | 981291 | 2014 | 7 صفحه PDF | دانلود رایگان |
25-Hydroxy-Grundmann’s ketone is a key building block in the chemical synthesis of vitamin D3 and its derivatives through convergent routes. Generally, the chemical synthesis of this compound involves tedious procedures and results in a mixture of several products. Recently, the selective hydroxylation of Grundmann’s ketone at position C25 by cytochrome P450 (CYP) 154E1 from Thermobifida fusca YX was described. In this study a recombinant whole-cell biocatalyst was developed and applied for hydroxylation of Grundmann’s ketone. Biotransformation was performed by Escherichia coli cells expressing CYP154E1 along with two redox partner systems, Pdx/PdR and YkuN/FdR. The system comprising CYP154E1/Pdx/PdR showed the highest production of 25-hydroxy-Grundmann’s ketone and resulted in 1.1 mM (300 mg L−1) product concentration.
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Journal: Bioorganic & Medicinal Chemistry - Volume 22, Issue 20, 15 October 2014, Pages 5586–5592