کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1357882 981302 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH
چکیده انگلیسی

We herein describe a novel in-stem molecular beacon (ISMB) containing multiple Cy3-quencher pairs on d-threoninol scaffolds in the stem region. The designed Cy3 derivative was not significantly quenched by the adjacent nucleobases, self-quenching of the fluorophore was minimal, and the fluorophore did not severely destabilize the duplex. Using newly designed Cy3, we synthesized ISMBs containing two Cy3 moieties. The signal to background ratio of the ISMB containing two Cy3 moieties was above 100, whereas that with one Cy3 was 30. A Cy3-derivative containing ISMB used in a fluorescence in situ hybridization (FISH) detected endogenous β-actin mRNA in fixed cells without need for washing procedures.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 23, Issue 8, 15 April 2015, Pages 1758–1762
نویسندگان
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