کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1361005 981455 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: The transferrin case
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: The transferrin case
چکیده انگلیسی

For detection of biological events in vitro, sensors using hyperpolarized 129Xe NMR can become a powerful tool, provided the approach can bridge the gap in sensitivity. Here we propose constructs based on the non-selective grafting of cryptophane precursors on holo-transferrin. This biological system was chosen because there are many receptors on the cell surface, and endocytosis further increases this density. The study of these biosensors with K562 cell suspensions via fluorescence microscopy and 129Xe NMR indicates a strong interaction, as well as interesting features such as the capacity of xenon to enter the cryptophane even when the biosensor is endocytosed, while keeping a high level of polarization. Despite a lack of specificity for transferrin receptors, undoubtedly due to the hydrophobic character of the cryptophane moiety that attracts the biosensor into the cell membrane, these biosensors allow the first in-cell probing of biological events using hyperpolarized xenon.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 13, 1 July 2011, Pages 4135–4143
نویسندگان
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