Synthesis of bicyclic molecular scaffolds (BTAa): An investigation towards new selective MMP-12 inhibitors

Starting from 3-aza-6,8-dioxa-bicyclo[3.2.1]octane scaffold (BTAa) a virtual library of molecules was generated and screened in silico against the crystal structure of the Human Macrophage Metalloelastase (MMP-12). The molecules obtaining high score were synthesized and the affinity for the catalytic domain of MMP-12 was experimentally proved by NMR experiments. A BTAa scaffold 20 having a N-hydroxyurea group in position 3 and a p-phenylbenzylcarboxy amide in position 7 showed a fair inhibition potency (IC50 = 149 μM) for MMP-12 and some selectivity towards five different MMPs. These results, taken together with the X-ray structure of the adduct between MMP-12, the inhibitor 20 and the acetohydroxamic acid (AHA), suggest that bicyclic scaffold derivatives may be exploited for the design of new selective matrix metalloproteinase inhibitors (MMPIs).

A selective MMP-12 inhibitor based on 3-aza-6,8-dioxa-bicyclo[3.2.1]octane-7-carboxylic acid (BTAa) is described. The observed inhibitory activity and the structural information on protein/inhibitor complexes provided by NMR experiments and X-ray assessments suggest that bicyclic scaffold derivatives (BTAa) may be exploited for the design of new selective MMP inhibitors.Figure optionsDownload as PowerPoint slide