کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1368969 981737 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506
چکیده انگلیسی

Solid materials for affinity resins bearing long PEG spacers between a functional group used for immobilization of a bio-active compound and the solid surface were synthesized to capture not only small target proteins but also large and/or complex target proteins. Solid materials with PEG1000 or PEG2000 as spacers, which bear a benzenesulfonamide derivative, exhibited excellent selectivity between the specific binding protein carbonic anhydrase type II (CAII) and non-specific ones. These materials also exhibited efficacy in capturing a particular target at a maximal amount. Affinity resins using solid materials with PEG1000 or PEG2000 spacers, bear a FK506 derivative, successfully captured the whole target complex of specific binding proteins at the silver staining level, while all previously known affinity resins with solid materials failed to achieve this objective. These novel affinity resins captured other specific binding proteins such as dynamin and neurocalcin δ as well.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 25, Issue 14, 15 July 2015, Pages 2788–2792
نویسندگان
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