کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1374497 | 1500617 | 2016 | 7 صفحه PDF | دانلود رایگان |
• 6-Amino-6-deoxy-curdlan is PEGylated and characterized.
• PEGylated 6-amino-6-deoxy-curdlan shows high affinity to short interfering RNA.
• PEGylated 6-amino-6-deoxy-curdlan is biocompatible.
• Fluorescein labeled PEGylated 6-amino-6-deoxy-curdlan distributes in mouse liver.
• PEGylated 6-amino-6-deoxy-curdlan efficiently delivers siRNA to mouse liver.
RNA interference (RNAi) is an evolutionarily conserved gene-silencing phenomenon that shows great promise for developing new therapies. However, the development of small interfering RNA (siRNA)-based therapies need to establish efficient delivery system that silences target genes with siRNA doses that is clinically feasible in humans. Here we report synthesis and in vivo study of a novel PEGylated curdlan-based nanoparticle, designated as 6AC-100PEG, obtained by conjugation of mPEG 2000 to 6-amino-6-deoxy-curdlan. The complex of siRNA/6AC-100PEG showed homogenous nanoparticles with an average diameter of 200 nm. MTT assay indicated that 6AC-100PEG does not have apparent cytotoxicity. Systemic administration of a complex of siapoB/6AC-100PEG significantly reduced the level of apoB mRNA in mouse liver, indicating that 6AC-100PEG can efficiently deliver siRNA to mouse liver and induce RNAi. Administration of siRNA/6AC-100PEG to mouse did not elevate liver enzyme level in the serum, indicating that 6AC-100PEG nanoparticle is a promising in vivo siRNA delivery agent.
Journal: Carbohydrate Polymers - Volume 141, 5 May 2016, Pages 92–98