کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1376086 1500658 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical analysis of expansin-like proteins from microbes
ترجمه فارسی عنوان
تجزیه و تحلیل بیوشیمیایی پروتئین هایی از قبیل اسپانسین از میکروب ها
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• Expansin-like proteins from three plant pathogenic bacteria and one fungus cause plant cell wall extension in vitro.
• Microbial expansins weaken filter paper networks without having detectable lytic activity.
• Microbial expansins do not act synergistically with selected cellulases for soluble sugar release from cellulose.

Expansins cause plant cell wall loosening and are present primarily in the plant kingdom. Gene sequence analysis suggests that expansins are present in several plant-colonizing or plant-pathogenic bacteria and fungi. However, experimental evidence of microbial expansin activity is largely lacking. Here we provide evidence that expansins from three plant pathogenic bacteria and one fungus cause extension of cell walls in vitro and weaken filter paper networks, without lytic activity. Since expansins were able to weaken cellulose networks, we tested whether they synergistically enhanced the activity of several cellulases in hydrolysis of cellulose. The microbial expansins did not show such synergism beyond the nonspecific effect of bovine serum albumin. Our results show that the expansins present in several pathogenic microbes have weak wall-loosening activity and we infer a role for these expansins in plant pathogenesis. Additionally, the convenient expression of several expansins in Escherichia coli makes a future comparative structure–function analysis among expansins possible in order to understand their activity at the molecular level.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 100, 16 January 2014, Pages 17–23
نویسندگان
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