D609 blocks cell survival and induces apoptosis in neural stem cells

In order to investigate the effects of tricyclodecane-9-yl-xanthogenate (D609) on the survival of neural stem cells (NSCs), which were isolated from rat forebrain, we treated the NSCs with D609 in the presence of basic fibroblast growth factor (bFGF). We found that when NSCs were exposed to 18.76–56.29 μM D609, the viability of the cells remarkably declined and apoptosis occurred. At the same time, the ROS level in NSCs was depressed. The data suggested that D609 was a powerful growth inhibitor and apoptosis inducer in NSCs.

D609 could suppress cell survival and induce apoptosis in rat neural stem cells. Moreover, the changes of intracellular ROS level induced by D609 indicated that a modest level of ROS might be indispensable to NSC survival.Figure optionsDownload as PowerPoint slide