کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1384025 982385 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Sodium periodate oxidized cotton yarn as carrier for immobilization of trypsin
چکیده انگلیسی

Cotton yarn was first oxidized by sodium periodate to introduce aldehyde groups which were able to react with amino groups of trypsin to form Schiff's base, and result in cotton yarn immobilized trypsin. The effect of periodate oxidation on the chemical and physical properties of cotton yarn was evaluated by determining aldehyde group content, fineness and tensile strength of yarn. Measurements of protein load from Bradford assay and catalytic activity in hydrolysis of N-α-benzoyl-dl-arginine p-nitroanilide were made for the immobilized enzyme. The maximum amount of immobilized trypsin was 6.1 mg/g dried cotton yarn. Trypsin immobilized on oxidized cotton yarn retained 90% and 72% of the initial activity at 4 °C and 25 °C, respectively, over 60 days of storage in physiological solution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 82, Issue 3, 15 October 2010, Pages 976–981
نویسندگان
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