کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1386428 982506 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assignment of the 1H and 13C NMR spectra of 2-aminobenzamide-labeled xylo-oligosaccharides
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Assignment of the 1H and 13C NMR spectra of 2-aminobenzamide-labeled xylo-oligosaccharides
چکیده انگلیسی

Xylose, xylo-oligosaccharides with a degree of polymerization (DP) from 2 to 6, xylo-oligosaccharides carrying a single arabinofuranosyl, glucosyluronic acid, or 4-O-methyl glucosyluronic acid residues and 4-β-d-Xylp-(1 → 4)-d-Xylp-(1 → 3)-α-l-Rhap-(1 → 2)-α-d-GalpA-(1 → 4)-d-Xylp were labeled at their reducing ends with 2-aminobenzamide (2AB) in the presence of sodium cyanoborohydride (NaBH3CN). These derivatives were then analyzed by high-performance anion-exchange chromatography (HPAEC) and structurally characterized by electrospray-ionization mass spectrometry (ESI-MS) and by 1H and 13C NMR spectroscopy. Reacting Xyl3-Xylitol-2AB with UDP-Xyl in the presence of rice microsomes resulted in the formation of small amounts of Xyl4–6-Xylitol-2AB showing that the 2AB-labeled compound is an acceptor for xylosyltransferase. The 2AB-labeled xylo-oligosaccharides and the 2AB-labeled xylo-oligosaccharides carrying 4-O-methyl glucuronic acid are fragmented by xylanase and α-glucuronidase present in the culture filtrate of Fomitopsis palustris FFPRI 0507. Thus, the 2AB-labeled xylo-oligosaccharides are useful for studying enzymes involved in xylan degradation and biosynthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 74, Issue 3, 4 November 2008, Pages 579–589
نویسندگان
, , , , , ,