کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1388307 1500839 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glycoprotein labeling with click chemistry (GLCC) and carbohydrate detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Glycoprotein labeling with click chemistry (GLCC) and carbohydrate detection
چکیده انگلیسی


• A method for labeling or detecting glycans on intact glycoprotein.
• Based on enzymatic incorporation of clickable monosaccharide.
• Highly specific to target glycans due to the specificities of glycosyltransferases.
• Highly sensitive due to the nature of click chemistry.
• As convenient as Western blotting.

Molecular labeling and detection techniques are essential to research in life science. Here, a method for glycoprotein labeling/carbohydrate detection through glycan replacement, termed glycoprotein labeling with click chemistry (GLCC), is described. In this method, a glycoprotein is first treated with specific glycosidases to remove certain sugar residues, a procedure that creates acceptor sites for a specific glycosyltransferase. A ‘clickable’ monosaccharide is then installed onto these sites by the glycosyltransferase. This modified glycoprotein is then conjugated to a reporter molecule using a click chemistry reaction. For glycoproteins that already contain vacant glycosylation sites, deglycosylation is not needed before the labeling step. As a demonstration, labeling on fetal bovine fetuin, mouse immunoglobulin IgG and bacterial expressed human TNFα and TNFβ are shown. Compared to traditional ways of protein labeling, labeling at glycosylation sites with GLCC is considerably more specific and less likely to have adverse effects, and, when utilized as a method for carbohydrate detection, this method is also highly specific and sensitive.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 412, 14 August 2015, Pages 1–6
نویسندگان
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