کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1388313 | 1500839 | 2015 | 8 صفحه PDF | دانلود رایگان |

• Lipid A and O-antigen structures from P. entomophila was determined.
• The lipid A resembles that from other Pseudomonas.
• Lipid A has low inflammatory activity.
• O-antigen is novel and has a tetrasaccharide repeating unit.
• Only 6-deoxy-2acetamido l-sugars compose the O-chain.
The structure and the immunology of the lipopolysaccharide (LPS) of Pseudomonas entomophila, an entomopathogenic bacterium isolated from the fruit fly Drosophila melanogaster, was characterized. The O-antigen portion was established and resulted to be built up of a repetitive unit constituted by four monosaccharide residues, all L configured, all deoxy at C-6 and with an acetamido function at C-2:→3)-α-l-FucNAc-(1→4)-α-l-FucNAc-(1→3)-α-l-FucNAc-(1→3)-β-l-QuiNAc-(1→The structural analysis of lipid A, showed a mixture of different species. The diphosphorylated glucosamine backbone carries six fatty acids consistent with the composition C10:0 3(OH), C12:0 2(OH) and C12:0 3(OH), whereas other species differs by the number of phosphates and/or of fatty acids. The immunology experiments demonstrated that the LPS structure of P. entomophila displayed a low ability to engage the TLR4-mediated signaling correlated to a significant antagonistic activity toward hexa-acylated LPS structures.
Figure optionsDownload as PowerPoint slide
Journal: Carbohydrate Research - Volume 412, 14 August 2015, Pages 20–27