Smith degradation of the O-antigenic polysaccharide of Salmonella Dakar: structural studies of the products

Two different oligosaccharides were obtained from the Smith degradation of the O-polysaccharide isolated from the lipopolysaccharide of Salmonella Dakar. The structures of these oligosaccharides were investigated by chemical analysis, NMR spectroscopy and MALDI-TOF mass spectrometry. The following structures of these products were determined: α-d-GalpNAc-(1→4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→2)-threitol and Figure optionsDownload as PowerPoint slidewhere Quip3NAc is 3-acetamido-3,6-dideoxyglucose. The reaction products confirmed the structure of the repeating unit of the Salmonella Dakar O-polysaccharide reported previously [Kumirska, J.; Szafranek, J.; Czerwicka, M.; Paszkiewicz, M.; Dziadziuszko, H.; Kunikowska, D.; Stepnowski, P. Carbohydr. Res. 2007,342, 2138–2143].

Two different oligosaccharides: α-d-GalpNAc-(1→4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→2)-threitoland where Quip3NAc is 3-acetamido-3,6-dideoxyglucose, were obtained from the Smith degradation of the O-polysaccharide isolated from the lipopolysaccharide of Salmonella Dakar. This paper presents practical methods for isolating and determining the full structure of such compounds.Figure optionsDownload as PowerPoint slide