کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1390731 983132 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elimination of glucose contamination from adipocyte glycogen extracts
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Elimination of glucose contamination from adipocyte glycogen extracts
چکیده انگلیسی

Glycogen was quantified in rat adipocytes by isolation using conventional KOH digestion and ethanol precipitation, followed by hydrolysis and spectrophotometric assay of the glucose product. A concentration of 0.193 ± 0.020 μmol glucosyl units/106 cells was recorded. When this procedure was modified by including a 4 h incubation with glucose oxidase prior to glycogen hydrolysis, the glycogen concentration was found to be 0.055 ± 0.008 μmol glucosyl units/106 cells. Therefore in adipocytes, conventional glycogen assays give substantial overestimates due to incomplete removal of glucose during glycogen isolation. Contaminant glucose can be scavenged in a simple manner by incubation with glucose oxidase prior to glycogen hydrolysis.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 343, Issue 9, 7 July 2008, Pages 1486–1489
نویسندگان
, , ,