کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1396665 | 984188 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The influence of molecular mass of sulfated propylene glycol ester of low-molecular-weight alginate on anticoagulant activities The influence of molecular mass of sulfated propylene glycol ester of low-molecular-weight alginate on anticoagulant activities](/preview/png/1396665.png)
Sulfated propylene glycol ester of low-molecular-weight alginate (PSS) was fractionated by low-pressure gel permeation chromatography. Four fractions (PSS1–4) with different weight-averaged molecular mass (51.95, 25.62, 11.76 and 5.41 kD, respectively) were obtained and their structural characteristics were determined and compared by Fourier transform infrared and nuclear magnetic resonance spectroscopes. Their anticoagulant activities were also studied by evaluating their influence on the activated partial thromboplastin time, thrombin time, anti-thrombin and anti-FXa factor activities mediated by anti-thrombin III and heparin cofactor II. The results demonstrated that PSS1–4 had similar structural parameters, such as the degree and pattern of sulfation and backbone, but their molecular masses were different. The bioassay results suggested that the anticoagulation was molecular mass dependent. PSS1 and PSS2 strongly inhibited the activity of thrombin mediated by heparin cofactor II and anti-thrombin III, whereas PSS4 only showed a very weak anti-thrombin activity. PSS1–3 weakly inhibited the activity of coagulation factor Xa mediated only by anti-thrombin III while PSS4 lacked the anti-Xa activity.
Journal: European Polymer Journal - Volume 43, Issue 7, July 2007, Pages 3009–3015