کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1405478 | 1501744 | 2014 | 5 صفحه PDF | دانلود رایگان |
The site-specific pH is an experimental probe for assessing models of structural folding and function of a protein as well as protein–protein and protein–ligand interactions. It can be determined by various techniques such as NMR, FT-IR, fluorescence and EPR spectroscopy. The latter require the use of external labels, i.e., employ pH-dependent dyes and spin labels, respectively. In this contribution, we outline an approach to a label-free and site-specific method for determining the local pH using deep ultraviolet resonance Raman (UVRR) spectroscopic fingerprints of the aromatic amino acids histidine and tyrosine in combination with a robust algorithm that determines the pH value using three UVRR reference spectra and without prior knowledge of the pKapKa.
Journal: Journal of Molecular Structure - Volume 1073, 5 September 2014, Pages 77–81