Synthesis of dihydromyricetin–manganese (II) complex and interaction with DNA

Dihydromyricetin has many physiological functions and its metal complex could have better effects. DNA is very important in biological body, but little attention has been devoted to the relationship between dihydromyricetin–metal complex and the DNA. In this paper, dihydromyricetin–Mn (II) complex has been prepared and characterized using UV–vis absorption spectrophotometry, IR spectroscopy, elemental analysis, and thermal gravimetric analysis (TG-DTA Analysis). The interaction of dihydromyricetin–Mn (II) complex with DNA was investigated using UV–vis spectra, fluorescence measurements and viscosity measurements. The results indicate that the dihydromyricetin–manganese (II) complex can intercalate into the stacked base pairs of DNA with binding constant Kb = 5.64 × 104 M and compete with the strong intercalator ethidium bromide for the intercalative binding sites with Stern–Volmer quenching constant, Ksq = 1.16.

► We firstly study the structure of dihydromyricetin–manganese (II) complex.
► We firstly study the interaction of complexes with DNA.
► The DMY-Mn(II) complex can interact with DNA by intercalation.
► The intercalation of complex with DNA is strong with Ksq = 1.16, Kb = 5.64 × 104 M.