کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1411642 1501868 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence properties and conformational stability of the hemocyanin from Chinese mitten crab Eriocheir japonica sinensis (Decapoda, Grapsidae)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Fluorescence properties and conformational stability of the hemocyanin from Chinese mitten crab Eriocheir japonica sinensis (Decapoda, Grapsidae)
چکیده انگلیسی

Guanidinium chloride-induced unfolding of hemocyanin from Chinese mitten crab (Eriocheir japonica sinensis), has been studied by UV/vis, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques. The unfolding of hemocyanin induced by Guanidinium chloride (GuHCl) has been studied by representation of fluorescence data in terms of “phase diagram”. The conformational transition from the native state to a partially folded intermediate of this protein occurs in the range of GuHCl concentration (0–3.5 M), and the transition from the intermediate to the unfolded state of this protein occurs in the range of GuHCl concentration (3.5–6.0 M), indicating that hemocyanin unfolding follows a three-state model under such conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 920, Issues 1–3, 28 February 2009, Pages 454–458
نویسندگان
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