Effect of dentinal tubules and resin-based endodontic sealers on fracture properties of root dentin

ObjectiveTo investigate the role of dentinal tubules in the fracture properties of human root dentin and whether resin-filled dentinal tubules can enhance fracture resistance.Materials and methodsCrack propagation in human root dentin was investigated in 200 μm thick longitudinal samples and examined by light and scanning electron microscopy. 30 maxillary premolar teeth were prepared for work of fracture (Wf) test at different tubule orientations, one perpendicular and two parallel to dentinal tubules. Another 40 single canal premolars were randomly divided into four groups of 10 each: intact dentin, prepared but unobturated canal, canal obturated with epoxy rein (AH Plus™/gutta percha), or with UDMA resin sealer (Resilon®/RealSeal®). The samples were prepared for Wf test parallel to dentinal tubules. Wf was compared under ANOVA with statistical significance set at p < 0.05.ResultsDentinal tubules influenced the path of cracks through dentin, with micro-cracks initiated in peritubular dentin of individual tubules ahead of the main crack tip. A significant difference (p < 0.001) was found between Wf perpendicular to tubule direction (254.9 J/m2) vs. parallel to tubule direction from inner to outer dentin (479.4 J/m2). Neither canal preparation nor obturation using epoxy- or UDMA-based resins as sealer cements substantially influenced fracture properties of root dentin, despite extensive infiltration of dentinal tubules by both sealer cements.