کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1422774 986462 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts
چکیده انگلیسی

SummaryObjectivesOral and systemic cells are permanently exposed to various types of xenobiotics, such as dental restorative materials, which may subsequently cause adverse effects. Objective of the present investigation was to analyze the effects of three important resin monomers on the glutathione metabolism of human gingival fibroblasts after an incubation period of 4 h.MethodsCells were exposed to various concentrations of 2-hydroxyethyl methacrylate (HEMA; 0.1–10 mM), triethylene-glycol dimethacrylate (TEGDMA; 0.05–2.5 mM), and urethane dimethacrylate (UDMA; 0.005–0.25 mM). Subsequently, cellular glutathione (GSH) concentrations were determined after a treatment period of 4 h using the monobromobimane assay. Data were statistically evaluated using Tukey ANOVA with p<0.05.ResultsGSH depletion was dependent on the type of the resin monomer: UDMA>TEGDMA>HEMA. The concentrations for a 50%-reduction of cellular GSH varied between 0.1 mM (0.05 mM) (UDMA), 0.33 mM (0.09 mM) (TEGDMA), and 1.6 mM (0.8 mM) (HEMA). Simultaneously, no decrease of cell numbers was found at any tested concentration.SignificanceThese data indicate that the investigated resins may cause cell damage due to depletion of intracellular GSH level even at low concentrations within a short period of time. The decrease of GSH is an early reaction, which is triggered prior to other cytotoxic alterations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dental Materials - Volume 22, Issue 6, June 2006, Pages 499–505
نویسندگان
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