کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1425514 | 986768 | 2010 | 7 صفحه PDF | دانلود رایگان |
An alternative vaccine delivery system for needle injections is the Bioneedle. Hepatitis B surface antigen (HBsAg) was formulated with Bioneedles. Three formulations were used: plain antigen, HBsAg adjuvated with aluminum hydroxide and HBsAg with LPS-derived lpxL1. Bioneedles with HBsAg-lpxL1 were the most stable and the most immunogenic formulations. The conventional liquid alum adjuvated vaccine lost 40% of its antigenicity after 1 week at 50 °C whereas the HBsAg-lpxL1 Bioneedles showed no significant decrease after 3 weeks at 50 °C. In vivo studies revealed that the HBsAg-lpxL1 Bioneedle formulations induced comparable IgG titers as conventional liquid formulations after 2 immunizations, but higher IgG2a titers were found already after 1 immunization. The in vivo and in vitro studies showed that the Bioneedle is an attractive alternative for needle injections of HBsAg vaccines.
Hepatitis B formulated with Bioneedles is an attractive alternative for conventional needle injections.Figure optionsDownload as PowerPoint slide
Journal: Journal of Controlled Release - Volume 147, Issue 2, 15 October 2010, Pages 211–217