Spatial regulation of specific gene expression through photoactivation of RNAi

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Photo-dependent gene silencing using several dyes, photopatterned RNAi, and single-cell RNAi was demonstrated.Figure optionsDownload as PowerPoint slide