کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1426197 | 986799 | 2009 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Controlled delivery of VEGF via modulation of alginate microparticle ionic crosslinking Controlled delivery of VEGF via modulation of alginate microparticle ionic crosslinking](/preview/png/1426197.png)
Clinical application of therapeutic angiogenesis is hampered by a lack of viable systems that demonstrate controlled, sustained release of vascular endothelial growth factor (VEGF). Alginate has emerged as a popular material for VEGF delivery; however most alginate-based systems offer limited means to control the rate of VEGF release beyond reducing the VEGF:alginate ratio to suboptimal efficiency. This study describes methods to control the release of VEGF from small (< 10 μm mean diameter) alginate microparticles via the use of different ionic crosslinkers. Crosslinking with Zn2+ versus Ca2+ reduced VEGF diffusional release and the combination of discrete populations of either Zn2+- or Ca2+-crosslinked particles allowed for control over the sustained release profiles for VEGF. The particle preparations were non-toxic and VEGF was bioactive after release. These results demonstrate that ionic modulation of alginate crosslinking is a viable strategy for controlling release of VEGF while retaining the high protein:polymer ratio that makes alginate an attractive carrier for delivery of protein therapeutics.
Journal: Journal of Controlled Release - Volume 134, Issue 1, 20 February 2009, Pages 26–34