کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1427272 | 986856 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cationized catalase-loaded hydrogel for growth inhibition of peritoneally disseminated tumor cells
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کلمات کلیدی
MMPIEPHBSSFBSICAM-1ROS - ROSHydrogen peroxide - آب اکسیژنهPeritoneal dissemination - انتشار پریتوئنControlled release - انتشار کنترل شدهfetal bovine serum - سرم جنین گاوmatrix metalloproteinase - ماتریکس متالوپروتئینازintercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Isoelectric point - نقطه ایزوالکتریکGelatin hydrogel - هیدروژل ژلاتینH2O2 - هیدروژن پراکسیدCatalase - کاتالازCationization - کاتیونیزاسیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A previous study demonstrated that ethylenediamine-conjugated catalase (ED-catalase) inhibits peritoneal dissemination of tumor cells in mice. To increase its inhibitory effects by sustained release, a hydrogel formulation of ED-catalase was prepared using a biodegradable hydrogel consisting of an acidic gelatin with an isoelectric point of 5.0. Although intraperitoneally injected ED-catalase solution rapidly disappeared from the cavity, more than 10% of ED-catalase remained even at 14Â days after implantation of ED-catalase/hydrogel into the cavity. Then, the effect of ED-catalase/hydrogel on peritoneal dissemination of tumor cells was evaluated by measuring the luciferase activity of abdominal organs after intraperitoneal inoculation of colon26/Luc, a colon adenocarcinoma stably expressing luciferase. ED-catalase/hydrogel showed a significantly (PÂ <Â 0.05) greater effect on inhibiting the growth of tumor cells than ED-catalase solution, demonstrating the importance of the retention of ED-catalase within the cavity as far as inhibition is concerned. Serial in vivo images of luciferase activity revealed that the ED-catalase/hydrogel significantly (PÂ <Â 0.05) retarded the growth rate of tumor cells. Survival of tumor-bearing mice supported the findings obtained with the luminescence-based analyses. These findings indicate that the sustained release of ED-catalase from hydrogels into the cavity is highly effective in inhibiting the growth of peritoneally disseminated tumor cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 122, Issue 2, 26 September 2007, Pages 151-158
Journal: Journal of Controlled Release - Volume 122, Issue 2, 26 September 2007, Pages 151-158
نویسندگان
Kenji Hyoudou, Makiya Nishikawa, Mai Ikemura, Yuki Kobayashi, Adam Mendelsohn, Nobuhiko Miyazaki, Yasuhiko Tabata, Fumiyoshi Yamashita, Mitsuru Hashida,