کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1427631 1509088 2006 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of the transdermal transport of flurbiprofen and indomethacin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Characterization of the transdermal transport of flurbiprofen and indomethacin
چکیده انگلیسی

Transdermal permeation of two types of NSAIDs, [3H] flurbiprofen and [14C] indomethacin, was examined by use of the Ussing-type chamber method. We found that the transdermal permeability in the absorptive direction (Pabs) of [3H] flurbiprofen was significantly higher than that of [14C] indomethacin. A lower pH (5.0) on the epidermal side increased the accumulation and the Pabs of [3H] flurbiprofen (18-fold and 50-fold, respectively) and [14C] indomethacin (18-fold and 22-fold, respectively), compared with pH 7.4. Coadministration of unlabeled flurbiprofen and indomethacin increased Pabs of [3H] flurbiprofen and [14C] indomethacin, respectively, in a concentration-dependent manner. Similar high-affinity transport was also observed in the uptake of [3H] flurbiprofen by human epidermal keratinocytes (HEK001 cells). RT-PCR analysis revealed the expression of mRNA of numerous transporters including MRP, OATP, MCT and OCTN family members in hairless mouse skin, human skin and HEK001 cells. These findings support the novel hypothesis that transdermal permeation of NSAIDs is mediated by saturable transport mechanisms, which may be candidates as targets for transdermal delivery of drugs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 110, Issue 3, 21 February 2006, Pages 542–556
نویسندگان
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