کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1427770 1509087 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual release of proteins from porous polymeric scaffolds
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Dual release of proteins from porous polymeric scaffolds
چکیده انگلیسی

To create porous scaffolds releasing in a controlled and independent fashion two different proteins, a novel approach based on protein-loaded polymeric coatings was evaluated. In this process, two water-in-oil emulsions are forced successively through a prefabricated scaffold to create coatings, containing each a different protein and having different release characteristics. In a first step, a simplified three-layered system was designed with model proteins (myoglobin and lysozyme). Poly(ether–ester) multiblock copolymers were chosen as polymer matrix, to allow the diffusion of proteins through the coatings. The model system showed the independent release of the two proteins. The myoglobin release was tailored from a burst to a linear release still on-going after 60 days, while the lysozyme release rate was kept constant. Macro-porous scaffolds, with a porosity of 59 vol.%, showed the same ability to control the release rate of the model proteins independently. The relation between the coatings properties and their release characteristics were investigated with the use of a mathematical diffusion model based on Fick's second law. It confirmed that the multiple coated scaffolds are biphasic system, where each coating controls the release of the protein that it contains. This approach could be of value for tissue engineering applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 111, Issues 1–2, 10 March 2006, Pages 95–106
نویسندگان
, , , , , ,