Click modification of helical amylose by poly(l-lysine) dendrons for non-viral gene delivery

• The click modification of amylose by poly(l-lysine) dendrons was carried out.
• This modified amylose could condense plasmid pMSCV-GFP-PARK2 into nanocomplexes.
• This modified amylose exhibited much lower cytotoxicity than commercial polyethylenimine.
• This modified amylose could delivery efficiently plasmid pMSCV-GFP-PARK2 to 293T cells.

Although amylose as a naturally-occurring helical polysaccharide has been widely used for biomedical applications, few studies have dealt with its chemical modification for non-viral gene delivery. In this work, the click modification of amylose by poly(l-lysine) dendrons was carried out and then characterized by Fourier transform infrared spectroscopy, wide-angle X-ray diffraction and elemental analyses. Such a modified polysaccharide exhibited excellent ability to condense plasmid pMSCV-GFP-PARK2 into compact and spherical nanoparticles. Moreover, it displayed much lower cytotoxicity when compared to branched polyethylenimine (bPEI, 25 kDa), a commercially available gene vector. Similar to bPEI, it had a dose-dependent gene transfection activity in human embryonic kidney 293T cells, as observed by confocal laser scanning microscopy and flow cytometry. At each optimized N/P ratio, the percentage of transfected cells by this modified polysaccharide was found to be comparable to that by bPEI. Western blot and cell apoptosis analyses confirmed its effectiveness for the delivery of plasmid pMSCV-GFP-PARK2 to 293T cells.

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