کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1428433 | 1509173 | 2015 | 8 صفحه PDF | دانلود رایگان |
• A simple method is described for preparation of porous gelatin scaffolds.
• The scaffolds were crosslinked using a range of glutaraldehyde concentrations.
• The scaffolds were tested using physical, mechanical, and biological methods.
• The scaffolds showed porous structure with interconnected porous systems.
The current study presents an effective and simple strategy to obtain stable porous scaffolds from gelatin via a gas foaming method. The technique exploits the intrinsic foaming ability of gelatin in the presence of CO2 to obtain a porous structure stabilised with glutaraldehyde. The produced scaffolds were characterised using physical and mechanical characterisation methods. The results showed that gas foaming may allow the tailoring of the 3-dimensional structure of the scaffolds with an interconnected porous structure. To assess the effectiveness of the preparation method in mitigating the potential cytotoxicity risk of using glutaraldehyde as a crosslinker, direct and in-direct cytotoxicity assays were performed at different concentrations of glutaraldehyde. The results indicate the potential of the gas foaming method, in the preparation of viable tissue engineering scaffolds.
Journal: Materials Science and Engineering: C - Volume 48, 1 March 2015, Pages 63–70