Study of different carbon materials for amperometric enzyme biosensor development

Diamond-like carbon (DLC) electrodes which constitute a new research area in electrochemistry and glassy carbon (GC) electrodes were used as transducers for fabrication of glucose oxidase (GOD) biosensors. The amperometric signal of the enzyme electrode was due to the electro-oxidation of H2O2 generated in the enzyme layer. This work has shown that the detection limit of glucose on GOD/GC electrode is 20 μM while it is 50 μM on GOD/DLC electrode. The sensitivity of GOD/GC electrode decreases 4% after 8 days and we have good repeatability of measurements for GOD/DLC electrode in the same day.