کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
15287 1400 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In silico identification of conserved microRNAs and their target transcripts from expressed sequence tags of three earthworm species
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
In silico identification of conserved microRNAs and their target transcripts from expressed sequence tags of three earthworm species
چکیده انگلیسی

MicroRNAs are a recently identified class of small regulatory RNAs that target more than 30% protein-coding genes. Elevating evidence shows that miRNAs play a critical role in many biological processes, including developmental timing, tissue differentiation, and response to chemical exposure. In this study, we applied a computational approach to analyze expressed sequence tags, and identified 32 miRNAs belonging to 22 miRNA families, in three earthworm species Eisenia fetida, Eisenia andrei, and Lumbricus rubellus. These newly identified earthworm miRNAs possess a difference of 2–4 nucleotides from their homologous counterparts in Caenorhabditis elegans. They also share similar features with other known animal miRNAs, for instance, the nucleotide U being dominant in both mature and pre-miRNA sequences, particularly in the first position of mature miRNA sequences at the 5′ end. The newly identified earthworm miRNAs putatively regulate mRNA genes that are involved in many important biological processes and pathways related to development, growth, locomotion, and reproduction as well as response to stresses, particularly oxidative stress. Future efforts will focus on experimental validation of their presence and target mRNA genes to further elucidate their biological functions in earthworms.

Figure optionsDownload as PowerPoint slideResearch highlights▶ We identified 32 miRNAs belonging to 22 miRNA families, in three earthworm species Eisenia fetida, Eisenia andrei, and Lumbricus rubellus. ▶ These newly identified earthworm miRNAs possess a difference of 2–4 nucleotides from their homologous counterparts in Caenorhabditis elegans. ▶ These earthworm miRNAs putatively regulate mRNA genes that are involved in many important biological processes and pathways related to development, growth, locomotion, and reproduction as well as response to stresses, particularly oxidative stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computational Biology and Chemistry - Volume 34, Issues 5–6, December 2010, Pages 313–319
نویسندگان
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