کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
15411 1411 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Statistical models in assessing fold change of gene expression in real-time RT-PCR experiments
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Statistical models in assessing fold change of gene expression in real-time RT-PCR experiments
چکیده انگلیسی

Real-time RT-PCR has been frequently used in quantitative research in molecular biology and bioinformatics. It provides remarkably useful technology to assess expression of genes. Although mathematical models for gene amplification process have been studied, statistical models and methods for data analysis in real-time RT-PCR have received little attention. In this paper, we briefly introduce current mathematical models, and study statistical models for real-time RT-PCR data. We propose a generalized estimation equations (GEE) model that properly reflects the structure of repeated data in RT-PCR experiments for both cross-sectional and longitudinal data. The GEE model takes the correlation between observations within the same subjects into consideration, and prevents from producing false positives or false negatives. We further demonstrate with a set of actual real-time RT-PCR data that different statistical models yield different estimations of fold change and confidence interval. The SAS program for data analysis using the GEE model is provided to facilitate easy computation for non-statistical professionals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computational Biology and Chemistry - Volume 30, Issue 1, February 2006, Pages 21–26
نویسندگان
, , , , ,