کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
15462 1415 2006 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An integrated model of glucose and galactose metabolism regulated by the GAL genetic switch
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
An integrated model of glucose and galactose metabolism regulated by the GAL genetic switch
چکیده انگلیسی

Glucose and galactose are two alternative carbon sources in yeast for energy production, producing CO2 and alcohol. The yeast needs to switch from glucose to galactose metabolism as required, by transcriptional regulation of the respective metabolic enzymes. This regulation is achieved mainly through the GAL genetic switch, in addition to glucose repression mechanism. This study integrates the two metabolic pathways with the genetic regulatory circuit using the GEPASI 3.30 simulation environment, and investigates the model behavior under various nutrient conditions. Our system is successful in achieving transcriptional upregulation of the galactose metabolizing enzymes as required. Under high glucose and high galactose concentrations, the in silico yeast chooses to metabolize glucose first, after which it resorts to using the galactose available. We also show what the preferred storage macromolecules are in different metabolic pathways.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computational Biology and Chemistry - Volume 30, Issue 3, June 2006, Pages 179–192
نویسندگان
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