کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1696760 | 1519141 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microcalorimetric studies of the effects of MgCl2 concentrations and pH on the adsorption of DNA on montmorillonite, kaolinite and goethite
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
ژئوشیمی و پترولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study attempted to comprehend the interaction mechanisms between DNA and the common minerals in soil such as montmorillonite, kaolinite and goethite at various environmental conditions. The effects of MgCl2 concentrations (0, 1, 10 and 60 mM) and pH (3.0, 5.0, 7.0 and 9.0) on the adsorption of DNA on the examined minerals were investigated by the equilibrium adsorption and direct measurement of adsorption enthalpies. The adsorption isotherms fitted by the Langmuirian model revealed that the maximum capacities and affinities of DNA adsorption on minerals increased with MgCl2 concentrations and decrease of pH. No DNA was adsorbed on montmorillonite at pH 9.0. The values of DNA adsorption enthalpies (ÎHads) ranged from â0.3 to 4.9 kJ gâ 1 at different systems. The ÎHads values decreased with increasing MgCl2 concentrations and decreasing pH. The adsorption of DNA on montmorillonite, kaolinite and goethite was an exothermic reaction (â0.3 < ÎHads < â 0.1 kJ gâ 1) at 60 mM MgCl2 and pH 3.0, suggesting a more significant electrostatic attraction in the adsorption process. In contrast, DNA adsorption on minerals became endothermic (0.1 < ÎHads < 4.9 kJ gâ 1) at 0-10 mM MgCl2 and pH 5.0-9.0 and dehydration effects were considered as the dominant driving forces for DNA adsorption on minerals. The thermodynamic parameters presented in this study have important implications for clarifying the binding mechanisms between DNA and mineral particles in soil and associated environments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Clay Science - Volume 32, Issues 1â2, April 2006, Pages 147-152
Journal: Applied Clay Science - Volume 32, Issues 1â2, April 2006, Pages 147-152
نویسندگان
Peng Cai, Qiaoyun Huang, Xuewen Zhang,