کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1760584 1019609 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Relationship Between Plasmid Size and Shock Wave-Mediated Bacterial Transformation
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم آکوستیک و فرا صوت
پیش نمایش صفحه اول مقاله
Relationship Between Plasmid Size and Shock Wave-Mediated Bacterial Transformation
چکیده انگلیسی
Bacterial transformation is a fundamental tool in molecular biology; nevertheless, there is still a lack of efficient methods for gene delivery. The use of shock waves has been proposed as an alternative. Recently, our group demonstrated that shock wave-induced transfer of deoxyribonucleic acid (DNA) into bacteria can be increased by enhancing acoustic cavitation; however, so far, little information exists about the effects of shock waves on DNA. The objective of this study was to identify the size regimes of plasmids (DNA molecules that are separate from the chromosomal DNA), which promote shock wave-induced transformation. The transformation efficiency of shock waves and the integrity of DNA were studied for six different plasmid sizes, using the parameters that led to the best results in our previous study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 38, Issue 6, June 2012, Pages 1078-1084
نویسندگان
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