Velocity Acceleration as a Determinant of Flow-Mediated Dilation

Shear stress is the established stimulus for flow-mediated dilation (FMD). In vivo, shear stress is typically estimated using mean blood velocity. However, mean blood velocity may not adequately characterize the shear stimulus. Pulsatile flow results in large shear gradients (velocity acceleration) at the onset of flow. The purpose of this study was to determine the importance of velocity acceleration to FMD. We define FMD as the brachial artery shear rate-diameter slope. Fourteen physically active, young (26 ± 5 years), male subjects were tested. Progressive forearm heating and handgrip exercise elicited steady-state increases in shear rate. FMD was measured prior to and following induced increases in velocity acceleration. Velocity acceleration was increased by inflating a tourniquet around the forearm to 40 mm Hg. Hierarchical linear modeling was used to estimate change in diameter with repeated measures of shear stress nested within each subject. Averaged across conditions, the 40 mm Hg cuff resulted in a 14% increase in velocity acceleration (p = 0.001). FMD was attenuated by 11.0% (p = 0.015) for the acceleration vs. control condition. However, after specifying velocity acceleration as a covariate, FMD was no longer significantly (p = 0.619) different between acceleration and control conditions. This finding suggests that mean blood velocity alone may not adequately characterize the shear stimulus.